Uncoupling of transcriptomic and cytological differentiation in mouse spermatocytes with impaired meiosis.
Mol Biol Cell 2019 Mar 1; 30(5):717-728
Cell differentiation is driven by changes in gene expression that manifest as changes in cellular phenotype or function. Altered cellular phenotypes, stemming from genetic mutations or other perturbations, are widely assumed to directly correspond to changes in the transcriptome and vice versa. Here, we exploited the cytologically well-defined Prdm9 mutant mouse as a model of developmental arrest to test whether parallel programs of cellular differentiation and gene expression are tightly coordinated, or can be disassociated. By comparing cytological phenotype markers and transcriptomes in wild-type and mutant spermatocytes, we identified multiple instances of cellular and molecular uncoupling in Prdm9
Fine, Alexander D; Ball, Robyn L; Fujiwara, Yasuhiro; Handel, Mary Ann; and Carter, Gregory W., "Uncoupling of transcriptomic and cytological differentiation in mouse spermatocytes with impaired meiosis." (2019). Faculty Research 2019. 54.