Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human Pancreatic β Cells for In Vitro and In Vivo Analysis.
Document Type
Article
Publication Date
3-5-2019
Keywords
JMG
Volume
29
Issue
3
First Page
745
Last Page
754
ISSN
1932-7420
PMID
30449685
DOI
https://doi.org/10.1016/j.cmet.2018.10.007
Grant
CA68485
Abstract
Identification of cell-surface markers specific to human pancreatic β cells would allow in vivo analysis and imaging. Here we introduce a biomarker, ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase3), that is expressed on the cell surface of essentially all adult human β cells, including those from individuals with type 1 or type 2 diabetes. NTPDase3 is expressed dynamically during postnatal human pancreas development, appearing first in acinar cells at birth, but several months later its expression declines in acinar cells while concurrently emerging in islet β cells. Given its specificity and membrane localization, we utilized an NTPDase3 antibody for purification of live human β cells as confirmed by transcriptional profiling, and, in addition, for in vivo imaging of transplanted human β cells. Thus, NTPDase3 is a cell-surface biomarker of adult human β cells, and the antibody directed to this protein should be a useful new reagent for β cell sorting, in vivo imaging, and targeting.
Recommended Citation
Saunders D,
Brissova M,
Phillips N,
Shrestha S,
Walker J,
Aramandla R,
Poffenberger G,
Flaherty D,
Weller K,
Pelletier J,
Cooper T,
Goff M,
Virostko J,
Shostak A,
Dean E,
Greiner D,
Shultz LD,
Prasad N,
Levy S,
Carnahan R,
Dai C,
Sévigny J,
Powers A.
Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human Pancreatic β Cells for In Vitro and In Vivo Analysis. . 2019; 29(3):745