Title

Creation of PDX-Bearing Humanized Mice to Study Immuno-oncology.

Document Type

Article

Publication Date

2019

JAX Source

Methods Mol Biol 2019; 1953:241-252

PMID

30912026

DOI

https://doi.org/10.1007/978-1-4939-9145-7_15

Abstract

A significant obstacle to the study of human cancer biology and the testing of human specific immunotherapeutics is the paucity of translational models that recapitulate both the growth of human tumors and the functionality of human immune systems. Humanized mice engrafted with human hematopoietic stem cells (HSC) and patient-derived xenografts (PDX) enable preclinical investigation of the interactions between the human immune system and human cancer. We use immunodeficient non-obese diabetic (NOD, scid, gamma) NSG™ or NSG™-SGM3 mice as hosts for establishment of human immunity following HSC injection and for engraftment of human tumors. Here we describe a refined protocol for the subcutaneous implant of solid PDX tumors into humanized mice. Protocols to recover infiltrating immune cells from growing tumors and to evaluate the immune cell subsets by flow cytometry are also described.

Comments

We thank Justin Jarvi and Marta Tewodros for trocar method development and technical assistance. This work was supported by The Jackson Laboratory.

Please contact the Joan Staats Library for information regarding this document.

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