The Loss of TBK1 Kinase Activity in Motor Neurons or in All Cell Types Differentially Impacts ALS Disease Progression in SOD1 Mice.
Document Type
Article
Publication Date
6-3-2020
Keywords
JMG
JAX Source
Neuron 2020 Jun 3; 106(5):789-805.e5
Volume
106
Issue
5
First Page
789
Last Page
805
ISSN
1097-4199
PMID
32220666
DOI
https://doi.org/10.1016/j.neuron.2020.03.005
Abstract
DNA sequence variants in the TBK1 gene associate with or cause sporadic or familial amyotrophic lateral sclerosis (ALS). Here we show that mice bearing human ALS-associated TBK1 missense loss-of-function mutations, or mice in which the Tbk1 gene is selectively deleted in motor neurons, do not display a neurodegenerative disease phenotype. However, loss of TBK1 function in motor neurons of the SOD1G93A mouse model of ALS impairs autophagy, increases SOD1 aggregation, and accelerates early disease onset without affecting lifespan. By contrast, point mutations that decrease TBK1 kinase activity in all cells also accelerate disease onset but extend the lifespan of SOD1 mice. This difference correlates with the failure to activate high levels of expression of interferon-inducible genes in glia. We conclude that loss of TBK1 kinase activity impacts ALS disease progression through distinct pathways in different spinal cord cell types and further implicate the importance of glia in neurodegeneration.
Recommended Citation
Gerbino V,
Kaunga E,
Ye J,
Canzio D,
O'Keeffe S,
Rudnick N,
Guarnieri P,
Lutz C,
Maniatis T.
The Loss of TBK1 Kinase Activity in Motor Neurons or in All Cell Types Differentially Impacts ALS Disease Progression in SOD1 Mice. Neuron 2020 Jun 3; 106(5):789-805.e5