Mapping systemic lupus erythematosus heterogeneity at the single-cell level.
Nat Immunol 2020 Sep; 21(9):1094-1106
Patients with systemic lupus erythematosus (SLE) display a complex blood transcriptome whose cellular origin is poorly resolved. Using single-cell RNA sequencing, we profiled ~276,000 peripheral blood mononuclear cells from 33 children with SLE with different degrees of disease activity and 11 matched controls. Increased expression of interferon-stimulated genes (ISGs) distinguished cells from children with SLE from healthy control cells. The high ISG expression signature (ISG
Nehar-Belaid, Djamel; Hong, Seunghee; Marches, Radu; Chen, Guo; Bolisetty, Mohan; Baisch, Jeanine; Walters, Lynnette; Punaro, Marilynn; Rossi, Robert J; Chung, Cheng-Han; Huynh, Richie P; Singh, Prashant; Flynn, William F; Tabanor-Gayle, Joy-Ann; Kuchipudi, Navya; Mejias, Asuncion; Collet, Magalie A; Lucido, Anna Lisa; Palucka, Karolina; Robson, Paul; Lakshminarayanan, Santhanam; Ramilo, Octavio; Wright, Tracey; Pascual, Virginia; and Banchereau, Jacques, "Mapping systemic lupus erythematosus heterogeneity at the single-cell level." (2020). Faculty Research 2020. 166.