Genomic and Phenotypic Characterization of a Broad Panel of Patient-Derived Xenografts Reflects the Diversity of Glioblastoma.
Clin Cancer Res 2020; 26(5):1094-1104
PURPOSE: Glioblastoma is the most frequent and lethal primary brain tumor. Development of novel therapies relies on the availability of relevant preclinical models. We have established a panel of 96 glioblastoma patient-derived xenografts (PDX) and undertaken its genomic and phenotypic characterization.
EXPERIMENTAL DESIGN: PDXs were established from glioblastoma, IDH-wildtype (
RESULTS: PDXs recapitulate many key phenotypic and molecular features of patient tumors. Orthotopic PDXs show characteristic tumor morphology and invasion patterns, but largely lack microvascular proliferation and necrosis. PDXs capture common and rare molecular drivers, including alterations of
CONCLUSIONS: Our PDX panel captures the molecular heterogeneity of glioblastoma and recapitulates many salient genetic and phenotypic features. All models and genomic data are openly available to investigators.
Vaubel, Rachael A; Tian, Shulan; Remonde, Dioval; Schroeder, Mark A; Mladek, Ann C; Kitange, Gaspar J; Caron, Alissa; Kollmeyer, Thomas M; Grove, Rebecca; Peng, Sen; Carlson, Brett L; Ma, Daniel J; Sarkar, Gobinda; Evers, Lisa; Decker, Paul A; Yan, Huihuang; Dhruv, Harshil D; Berens, Michael E; Wang, Qianghu; Marin, Bianca M; Klee, Eric W; Califano, Andrea; LaChance, Daniel H; Eckel-Passow, Jeanette E; Verhaak, Roel G W; Sulman, Erik P; Burns, Terry C; Meyer, Fredrick B; O'Neill, Brian P; Tran, Nhan L; Giannini, Caterina; Jenkins, Robert B; Parney, Ian F; and Sarkaria, Jann N, "Genomic and Phenotypic Characterization of a Broad Panel of Patient-Derived Xenografts Reflects the Diversity of Glioblastoma." (2020). Faculty Research 2020. 60.