The X chromosome from telomere to telomere: key achievements and future opportunities.
Fac Rev 2021 Jul 30; 10:63
While the human genome represents the most accurate vertebrate reference assembly to date, it still contains numerous gaps, including centromeric and other large repeat-containing regions - often termed the "dark side" of the genome - many of which are of fundamental biological importance. Miga et al.1,2 present the first gapless assembly of the human X chromosome, with the help of ultra-long-read nanopore reads generated for the haploid complete hydatidiform mole (CHM13) genome. They reconstruct the ~3.1 megabase centromeric satellite DNA array and map DNA methylation patterns across complex tandem repeats and satellite arrays. This Telomere-to-Telomere assembly provides a superior human X chromosome reference enabling future sex-determination and X-linked disease research, and provides a path towards finishing the entire human genome sequence.
Heard, Edith; Johnson, Alexander D; Korbel, Jan O; Lee, Charles; Snyder, Michael P; and Sturgill, David, "The X chromosome from telomere to telomere: key achievements and future opportunities." (2021). Faculty Research 2021. 288.