Document Type
Article
Publication Date
1-14-2021
Publication Title
MicroPubl Biol
Keywords
JMG
JAX Source
MicroPubl Biol . 2021 Jan 14:2021:10.17912/micropub.biology.000353.
Volume
2021
First Page
682
Last Page
682
ISSN
2578-9430
PMID
33474525
DOI
10.17912/micropub.biology.000353
Grant
This work was supported, in part, by a grant W911NF-18-1-0465 from the Department of Defense to A.K.A. (L.M.F. and A.K.A.) and by grant 1R15HD084253-01A1 from the National Institutes of Health to A.K.A (K.G., R.B. and A.K.A). The spinning disk confocal microscope was acquired through a Department of Defense HBCU/MI Equipment/Instrumentation Grant (#64684-RT-REP) to A.K.A.
Abstract
We have generated a WEE-1.3 strain in C. elegans where we have endogenously tagged the C-terminus with GFP. In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show for the first time that endogenously tagging WEE-1.3 at either termini does not affect the reproductive function of the worms.
Recommended Citation
Fernando L,
Golliday K,
Boateng R,
Allen A.
Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in MicroPubl Biol . 2021 Jan 14:2021:10.17912/micropub.biology.000353.
Comments
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.