Document Type
Article
Publication Date
1-29-2021
Keywords
JGM
JAX Source
Commun Biol 2021 Jan 29; 4(1):129.
PMID
33514825
DOI
https://doi.org/10.1038/s42003-021-01649-6
Grant
AI121920; AI142733; NS105539
Abstract
Development of antibody protection during SARS-CoV-2 infection is a pressing question for public health and for vaccine development. We developed highly sensitive SARS-CoV-2-specific antibody and neutralization assays. SARS-CoV-2 Spike protein or Nucleocapsid protein specific IgG antibodies at titers more than 1:100,000 were detectable in all PCR+ subjects (n = 115) and were absent in the negative controls. Other isotype antibodies (IgA, IgG1-4) were also detected. SARS-CoV-2 neutralization was determined in COVID-19 and convalescent plasma at up to 10,000-fold dilution, using Spike protein pseudotyped lentiviruses, which were also blocked by neutralizing antibodies (NAbs). Hospitalized patients had up to 3000-fold higher antibody and neutralization titers compared to outpatients or convalescent plasma donors. Interestingly, some COVID-19 patients also possessed NAbs against SARS-CoV Spike protein pseudovirus. Together these results demonstrate the high specificity and sensitivity of our assays, which may impact understanding the quality or duration of the antibody response during COVID-19 and in determining the effectiveness of potential vaccines.
Recommended Citation
Dogan, Mikail; Kozhaya, Lina; Placek, Lindsey; Gunter, Courtney; Yigit, Mesut; Hardy, Rachel; Plassmeyer, Matthew; Coatney, Paige; Lillard, Kimberleigh; Bukhari, Zaheer; Kleinberg, Michael; Hayes, Chelsea; Arditi, Moshe; Klapper, Ellen; Merin, Noah; Liang, Bruce Tsan-Tang; Gupta, Raavi; Alpan, Oral; and Unutmaz, Derya, "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." (2021). Faculty Research 2021. 39.
https://mouseion.jax.org/stfb2021/39
Comments
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