Title

Flow cytometric identification of T fh 13 cells in mouse and human

Document Type

Article

Publication Date

2-2021

Keywords

JGM

JAX Source

J Allergy Clin Immunol 2021 Feb; 147(2):470-483

Volume

147

Issue

2

First Page

470

Last Page

483

ISSN

1097-6825

PMID

32709424

DOI

https://doi.org/10.1016/j.jaci.2020.04.063

Abstract

Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper Tfh13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying Tfh13 cells in both mice and humans.

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