Int J Mol Sci 2021 Feb 19; 22(4):2071
CA034196, GM104318, AG022308, AI145383, The Foundation Leducq Transatlantic Network of Excellence in Cardiac Research and the Director’s Innovation Fund at The Jackson Laboratory.
Recent technological advances have revolutionized the study of tissue biology and garnered a greater appreciation for tissue complexity. In order to understand cardiac development, heart tissue homeostasis, and the effects of stress and injury on the cardiovascular system, it is essential to characterize the heart at high cellular resolution. Single-cell profiling provides a more precise definition of tissue composition, cell differentiation trajectories, and intercellular communication, compared to classical bulk approaches. Here, we aim to review how recent single-cell multi-omic studies have changed our understanding of cell dynamics during cardiac development, and in the healthy and diseased adult myocardium.
Forte, Elvira; McLellan, Michael A; Skelly, Daniel A; and Rosenthal, Nadia, "Ex uno, plures-From One Tissue to Many Cells: A Review of Single-Cell Transcriptomics in Cardiovascular Biology." (2021). Faculty Research 2021. 66.