Identification of cell-surface glycans that mediate motility-dependent binding and internalization of Pseudomonas aeruginosa by phagocytes.
JMG, Cell Line, Tumor, Cells, Cultured, HL-60 Cells, Heparitin Sulfate, Humans, Immunity, Innate, Interleukin-1beta, Monocytes, Phagocytes, Phagocytosis, Polysaccharides, Pseudomonas Infections, Pseudomonas aeruginosa, THP-1 Cells
Mol Immunol 2021 Mar; 131:68-77
Phagocytic cells are critical to host defense against Pseudomonas aeruginosa, a Gram-negative bacterium that is an opportunistic pathogen. Accordingly, susceptible individuals frequently have impaired innate immune responses, including those with cystic fibrosis or neutropenia. Previous studies identified that the downregulation, or loss, of bacterial flagellar motility enables bacteria to evade interactions with phagocytic cells that result in phagocytic uptake of the bacteria. However, the mechanistic bases for motility-dependent interactions between P. aeruginosa and host cell surfaces that lead to phagocytic uptake of the bacteria are poorly understood. A recent insight is that exogenous addition of a negatively charged phospholipid, phosphatidylinositol-(3,4,5)-triphosphate (PIP
Sanchez, Hector; Hopkins, Daniel; Demirdjian, Sally; Gutierrez, Cecilia; O'Toole, George A; Neelamegham, Sriram; and Berwin, Brent, "Identification of cell-surface glycans that mediate motility-dependent binding and internalization of Pseudomonas aeruginosa by phagocytes." (2021). Faculty Research 2021. 69.