Document Type

Article

Publication Date

7-9-2022

Publication Title

Nat Commun

Keywords

JGM, Alleles, Animals, Cell Differentiation, Drosophila, Drosophila Proteins, Drosophila melanogaster, Germ Cells

JAX Source

Nat Commun 2022 Jul 9; 13(1):3981

Volume

13

Issue

1

First Page

3981

Last Page

3981

ISSN

2041-1723

PMID

35810185

DOI

https://doi.org/10.1038/s41467-022-31737-y

Grant

GM133562

Abstract

Pairing of homologous chromosomes in somatic cells provides the opportunity of interchromosomal interaction between homologous gene regions. In the Drosophila male germline, the Stat92E gene is highly expressed in a germline stem cell (GSC) and gradually downregulated during the differentiation. Here we show that the pairing of Stat92E is always tight in GSCs and immediately loosened in differentiating daughter cells, gonialblasts (GBs). Disturbance of Stat92E pairing by relocation of one locus to another chromosome or by knockdown of global pairing/anti-pairing factors both result in a failure of Stat92E downregulation, suggesting that the pairing is required for the decline in transcription. Furthermore, the Stat92E enhancer, but not its transcription, is required for the change in pairing state, indicating that pairing is not a consequence of transcriptional changes. Finally, we show that the change in Stat92E pairing is dependent on asymmetric histone inheritance during the asymmetric division of GSCs. Taken together, we propose that the changes in Stat92E pairing status is an intrinsically programmed mechanism for enabling prompt cell fate switch during the differentiation of stem cells.

Comments

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.

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