Single-cell transcriptome analysis defines mesenchymal stromal cells in the mouse incisor dental pulp.
Document Type
Article
Publication Date
2022
Publication Title
Gene expression patterns : GEP
Keywords
JGM
JAX Source
Gene Expr Patterns 2022; 43:119228
Volume
43
First Page
119228
Last Page
119228
ISSN
1872-7298
PMID
34915194
DOI
https://doi.org/10.1016/j.gep.2021.119228
Abstract
The dental pulp is known to be highly heterogenous, comprising distinct cell types including mesenchymal stromal cells (MSCs), which represent neural-crest-derived cells with the ability to differentiate into multiple cell lineages. However, the cellular heterogeneity and the transcriptome signature of different cell clusters within the dental pulp remain to be established. To better understand discrete cell types, we applied a single-cell RNA sequencing strategy to establish the RNA expression profiles of individual dental pulp cells from 5- to 6-day-old mouse incisors. Our study revealed distinct subclasses of cells representing osteoblast, odontoblast, endothelial, pancreatic, neuronal, immune, pericyte and ameloblast lineages. Collectively, our research demonstrates the complexity and diversity of cell subclasses within the incisor dental pulp, thus providing a foundation for uncovering the molecular processes that govern cell fate decisions and lineage commitment in dental pulp-derived MSCs.
Recommended Citation
Bayarsaihan D,
Enkhmandakh B,
Vijaykumar A,
Robson P,
Mina M.
Single-cell transcriptome analysis defines mesenchymal stromal cells in the mouse incisor dental pulp. Gene Expr Patterns 2022; 43:119228