Title

Single-cell transcriptome analysis defines mesenchymal stromal cells in the mouse incisor dental pulp.

Document Type

Article

Publication Date

2022

Publication Title

Gene expression patterns : GEP

Keywords

JGM

JAX Source

Gene Expr Patterns 2022; 43:119228

Volume

43

First Page

119228

Last Page

119228

ISSN

1872-7298

PMID

34915194

DOI

https://doi.org/10.1016/j.gep.2021.119228

Abstract

The dental pulp is known to be highly heterogenous, comprising distinct cell types including mesenchymal stromal cells (MSCs), which represent neural-crest-derived cells with the ability to differentiate into multiple cell lineages. However, the cellular heterogeneity and the transcriptome signature of different cell clusters within the dental pulp remain to be established. To better understand discrete cell types, we applied a single-cell RNA sequencing strategy to establish the RNA expression profiles of individual dental pulp cells from 5- to 6-day-old mouse incisors. Our study revealed distinct subclasses of cells representing osteoblast, odontoblast, endothelial, pancreatic, neuronal, immune, pericyte and ameloblast lineages. Collectively, our research demonstrates the complexity and diversity of cell subclasses within the incisor dental pulp, thus providing a foundation for uncovering the molecular processes that govern cell fate decisions and lineage commitment in dental pulp-derived MSCs.

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