Title

Derivation of extra-embryonic and intra-embryonic macrophage lineages from human pluripotent stem cells.

Document Type

Article

Publication Date

4-15-2022

Publication Title

Development (Cambridge, England)

Keywords

Cell Differentiation, Hematopoiesis, Homeostasis, Humans, Macrophages, Pluripotent Stem Cells

JAX Location

JGM

Volume

149

Issue

8

ISSN

1477-9129

PMID

35178561

DOI

https://doi.org/10.1242/dev.200016

Abstract

Tissue-resident macrophages are increasingly recognized as important determinants of organ homeostasis, tissue repair, remodeling and regeneration. Although the ontogeny and function of tissue-resident macrophages has been identified as distinct from postnatal hematopoiesis, the inability to specify, in vitro, similar populations that recapitulate these developmental waves has limited our ability to study their function and potential for regenerative applications. We took advantage of the concept that tissue-resident macrophages and monocyte-derived macrophages originate from distinct extra-embryonic and definitive hematopoietic lineages to devise a system to generate pure cultures of macrophages that resemble tissue-resident or monocyte-derived subsets. We demonstrate that human pluripotent stem cell-derived extra-embryonic-like and intra-embryonic-like hematopoietic progenitors differentiate into morphologically, transcriptionally and functionally distinct macrophage populations. Single-cell RNA sequencing of developing and mature cultures uncovered distinct developmental trajectories and gene expression programs of macrophages derived from extra-embryonic-like and intra-embryonic-like hematopoietic progenitors. These findings establish a resource for the generation of human tissue resident-like macrophages to study their specification and function under defined conditions and to explore their potential use in tissue engineering and regenerative medicine applications.

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