The dark kinase STK32A regulates hair cell planar polarity opposite of EMX2 in the developing mouse inner ear. Elife. 2023;12:e84910
JMG, Animals, Mice, Cell Polarity, Hair Cells, Auditory, Transcription Factors, Vestibule, Labyrinth, Sensory Receptor Cells
This work was supported by the National Institutes of Health (R01DC013066 to MRD, R01DC015242 and R01DC018304 to BT, and Eunice Kennedy Shriver National Institute of Child Health & Human Development of the NIH Award #T32HD007491 to EMR). We thank Doris Wu (NIDCD) for sharing reagents.
The vestibular maculae of the inner ear contain sensory receptor hair cells that detect linear acceleration and contribute to equilibrioception to coordinate posture and ambulatory movements. These hair cells are divided between two groups, separated by a line of polarity reversal (LPR), with oppositely oriented planar-polarized stereociliary bundles that detect motion in opposite directions. The transcription factor EMX2 is known to establish this planar polarized organization in mouse by regulating the distribution of the transmembrane receptor GPR156 at hair cell boundaries in one group of cells. However, the genes regulated by EMX2 in this context were previously not known. Using mouse as a model, we have identified the serine threonine kinase STK32A as a downstream effector negatively regulated by EMX2.