Document Type
Article
Publication Date
11-28-2024
Original Citation
Huy T,
Nguyen T,
Salad S,
Aguilar C,
Reyes A,
Arayan L,
Min W,
Lee H,
Hop H,
Kim S.
Hypertonic Saline Induces Host Protective Immune Responses against Microbiol Biotechnol. 2024;34(11):2192–200
Keywords
JMG, Animals, Brucellosis, Brucella abortus, Mice, Cytokines, Spleen, Saline Solution, Hypertonic, Th1 Cells, Mice, Inbred BALB C, Female, Liver, Nitric Oxide Synthase Type II, NF-kappa B, Disease Models, Animal, Autophagy
JAX Source
Microbiol Biotechnol. 2024;34(11):2192–200
ISSN
1738-8872
PMID
39403730
DOI
https://doi.org/10.4014/jmb.2407.07040
Abstract
Hypertonic saline (HTS) resuscitation can enhance immune responses against various pathogens, however, the effect of HTS on brucellosis is yet to be defined. In this study, we found that HTS inhibited Brucella infection in mice by augmenting Th1 immunity. HTS treatment enhanced the serum cytokines production and the expression of nitric oxide synthase (NOS2) and nuclear factor kappa B (NF-ĸB) p50 and p65, crucial anti-Brucella effectors in splenocytes. In addition, HTS treatment also inhibited the phosphorylation of MAPK signaling, accompanied by the down-regulation of the autophagy marker LC3B-II. Due to directing an appropriate immune response, HTS treatment substantially decreased bacterial burden in spleen and liver tissues. In summary, corroborating previous studies showing the antimicrobial effects of HTS, our findings indicate that HTS treatment triggers a protective immune response against Brucella infection. Additionally, these results provide promising evidence of the immunomodulatory role of HTS in controlling bacterial infections.
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This work is licensed under a Creative Commons Attribution 4.0 International License.