In vivo models of subclonal oncogenesis and dependency in hematopoietic malignancy.
Document Type
Article
Publication Date
11-11-2024
Original Citation
Bowman R,
Dunbar A,
Mishra T,
Xiao W,
Waarts M,
Maestre I,
Eisman S,
Cai L,
Mowla S,
Shah N,
Youn A,
Bennett L,
Fontenard S,
Gounder S,
Gandhi A,
Bowman M,
O'Connor K,
Zaroogian Z,
Sánchez-Vela P,
Martinez Benitez A,
Werewski M,
Park Y,
Csete I,
Krishnan A,
Lee D,
Boorady N,
Potts C,
Jenkins M,
Cai S,
Carroll M,
Meyer S,
Miles L,
Ferrell P,
Trowbridge JJ,
Levine R.
In vivo models of subclonal oncogenesis and dependency in hematopoietic malignancy. Cancer Cell. 2024;42(11):1955-69.e7.
Keywords
JMG, Animals, Mutation, Nucleophosmin, Humans, DNA Methyltransferase 3A, Mice, Carcinogenesis, fms-Like Tyrosine Kinase 3, Isocitrate Dehydrogenase, DNA (Cytosine-5-)-Methyltransferases, Hematologic Neoplasms, Cell Transformation, Neoplastic, Nuclear Proteins, Mutagenesis
JAX Source
Cancer Cell. 2024;42(11):1955-69.e7.
ISSN
1878-3686
PMID
39532065
DOI
https://doi.org/10.1016/j.ccell.2024.10.009
Grant
J.J.T. is a scholar of the Leukemia and Lymphoma Society and supported by U01AG077925.
Abstract
Cancer evolution is a multifaceted process leading to dysregulation of cellular expansion and differentiation through somatic mutations and epigenetic dysfunction. Clonal expansion and evolution is driven by cell-intrinsic and -extrinsic selective pressures, which can be captured with increasing resolution by single-cell and bulk DNA sequencing. Despite the extensive genomic alterations revealed in profiling studies, there remain limited experimental systems to model and perturb evolutionary processes. Here, we integrate multi-recombinase tools for reversible, sequential mutagenesis from premalignancy to leukemia. We demonstrate that inducible Flt3 mutations differentially cooperate with Dnmt3a, Idh2, and Npm1 mutant alleles, and that changing the order of mutations influences cellular and transcriptional landscapes. We next use a generalizable, reversible approach to demonstrate that mutation reversion results in rapid leukemic regression with distinct differentiation patterns depending upon co-occurring mutations. These studies provide a path to experimentally model sequential mutagenesis, investigate mechanisms of transformation and probe oncogenic dependency in disease evolution.