Structures of ATP-binding cassette transporter ABCC1 reveal the molecular basis of cyclic dinucleotide cGAMP export.

Document Type

Article

Publication Date

1-14-2025

Publication Title

Immunity

Keywords

JGM, Humans, Nucleotides, Cyclic, Multidrug Resistance-Associated Proteins, Cryoelectron Microscopy, Models, Molecular, Immunity, Innate, HEK293 Cells, Protein Binding, Binding Sites, Protein Multimerization

JAX Source

Immunity. 2025;58(1):59-73.

Volume

58

Issue

1

First Page

59

Last Page

73

ISSN

1097-4180

PMID

39765229

DOI

https://doi.org/10.1016/j.immuni.2024.12.002

Grant

HL148153 to A.P.W., and grant AI179168 to J.J.V.

Abstract

Cyclic nucleotide GMP-AMP (cGAMP) plays a critical role in mediating the innate immune response through the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway. Recent studies showed that ATP-binding cassette subfamily C member 1 (ABCC1) is a cGAMP exporter. The exported cGAMP can be imported into uninfected cells to stimulate a STING-mediated innate immune response. However, the molecular basis of cGAMP export mediated by ABCC1 remains unclear. Here, we report the cryoelectron microscopy (cryo-EM) structures of human ABCC1 in a ligand-free state and a cGAMP-bound state. These structures reveal that ABCC1 forms a homodimer via its N-terminal transmembrane domain. The ligand-bound structure shows that cGAMP is recognized by a positively charged pocket. Mutagenesis and functional studies confirmed the roles of the ligand-binding pocket in cGAMP recognition and export. This study provides insights into the structure and function of ABCC1 as a cGAMP exporter and lays a foundation for future research targeting ABCC1 in infection and anti-cancer immunity.

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