Tuning of human MAIT cell activation by commensal bacteria species and MR1-dependent T-cell presentation.

Cihan Tastan
Ece Karhan, The Jackson Laboratory
Wei Zhou, The Jackson Laboratory
Elizabeth Fleming, The Jackson Laboratory
Anita Y Voigt, The Jackson Laboratory
Xudong Yao
Lei Wang
Meghan Horne
Lindsey Placek, The Jackson Laboratory
Lina Kozhaya, The Jackson Laboratory
Julia Oh, The Jackson Laboratory
Derya Unutmaz, The Jackson Laboratory


Human mucosal-associated invariant T (MAIT) cell receptors (TCRs) recognize bacterial riboflavin pathway metabolites through the MHC class 1-related molecule MR1. However, it is unclear whether MAIT cells discriminate between many species of the human microbiota. To address this, we developed an in vitro functional assay through human T cells engineered for MAIT-TCRs (eMAIT-TCRs) stimulated by MR1-expressing antigen-presenting cells (APCs). We then screened 47 microbiota-associated bacterial species from different phyla for their eMAIT-TCR stimulatory capacities. Only bacterial species that encoded the riboflavin pathway were stimulatory for MAIT-TCRs. Most species that were high stimulators belonged to Bacteroidetes and Proteobacteria phyla, whereas low/non-stimulator species were primarily Actinobacteria or Firmicutes. Activation of MAIT cells by high- vs low-stimulating bacteria also correlated with the level of riboflavin they secreted or after bacterial infection of macrophages. Remarkably, we found that human T-cell subsets can also present riboflavin metabolites to MAIT cells in a MR1-restricted fashion. This T-T cell-mediated signaling also induced IFNγ, TNF and granzyme B from MAIT cells, albeit at lower level than professional APC. These findings suggest that MAIT cells can discriminate and categorize complex human microbiota through computation of TCR signals depending on antigen load and presenting cells, and fine-tune their functional responses.