Flow cytometric identification of T

Jennifer S Chen
Jessica D S Grassmann, The Jackson Laboratory
Uthaman Gowthaman
Sam J Olyha
Tregony Simoneau
M Cecilia Berin
Stephanie C Eisenbarth
Adam Williams, The Jackson Laboratory


Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper Tfh13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying Tfh13 cells in both mice and humans.