Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing.

Anna Thrun
Aitor Garzia
Yu Kigoshi-Tansho
Pratik R Patil
Charles S Umbaugh
Teresa Dallinger
Jia Liu
Sylvia Kreger
Annarita Patrizi
Gregory A. Cox, The Jackson Laboratory
Thomas Tuschl
Claudio A P Joazeiro


Incompletely synthesized nascent chains obstructing large ribosomal subunits are targeted for degradation by ribosome-associated quality control (RQC). In bacterial RQC, RqcH marks the nascent chains with C-terminal alanine (Ala) tails that are directly recognized by proteasome-like proteases, whereas in eukaryotes, RqcH orthologs (Rqc2/NEMF [nuclear export mediator factor]) assist the Ltn1/Listerin E3 ligase in nascent chain ubiquitylation. Here, we study RQC-mediated proteolytic targeting of ribosome stalling products in mammalian cells. We show that mammalian NEMF has an additional, Listerin-independent proteolytic role, which, as in bacteria, is mediated by tRNA-Ala binding and Ala tailing. However, in mammalian cells Ala tails signal proteolysis indirectly, through a pathway that recognizes C-terminal degrons; we identify the CRL2