The Effects of Genetic Variation on Acute Myeloid Leukemia Growth in Mice


Dan Bernstein

Document Type


Publication Date

Summer 2022



JAX Location

In: Student Reports, Summer 2022, The Jackson Laboratory


The tumor microenvironment is a term used to describe the makeup of the tissues surrounding a tumor. It affects how many resources are available to the cancer cells, what stressors they endure, and the overall conditions for their growth. In this project, I investigated the effect of genetically different microenvironments on Acute Myeloid Leukemia (AML) growth. To model tumor growth, I engrafted C1498 line of murine AML cells into different strains of immunodeficient mice. Growth was quantified using flow cytometry and immunohistochemistry of mouse samples. Tumor localization was observed by labelling cancer cells with fluorescent dye before engraftment. This line of cancer grew best when engrafted into its syngeneic environment compared to genetically different allogenic mice. This research shows that the tumor’s microenvironment plays a significant role in cancer growth and needs to be considered when doing future research.

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