Chromatin Looping Differences between 129S and CAST Mice Regulate Transcriptional Variation


Beatriz Alvarez

Document Type


Publication Date

Summer 2022



JAX Location

In: Student Reports, Summer 2022, The Jackson Laboratory


The histone protein tails are subject to post-translational modifications (PTMs) that are an important feature of gene regulation and genome organization (Musselman et al, 2012). PTMs are epigenetic markers that can be inherited. Epigenomics is a field of study focused on how PTMs and other DNA binding factors contribute to gene regulation without altering the DNA sequence (Gibney and Nolan, 2010). This study will help characterize the epigenomic landscape around strain-specific looping events and help identify factors that drive differential gene transcription. We want to identify the inheritance pattern of histone modifications in parental and F1 hybrid mouse strain. Chromatin Immunoprecipitation (ChIP-Seq) will be applied in mouse embryonic stem cells (mESCs) lines against histone modifications: H3K27ac and H3K27me3, and chromatin looping proteins: CTCF and RNAPII. The 129S1xCAST data hints at which parent the epigenome was inherited from and allows us to predict transcriptional levels in the F1 hybrid.

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