Faculty Research 1970 - 1979

Control of proliferation and differentiation in B lymphocytes by anti-Ig antibodies and a serum-derived cofactor.

Document Type

Article

Publication Date

1978

Keywords

Animals-Newborn: im, Anti-Antibodies, Antibody-Formation, B-Lymphocytes: im, Blood-Proteins, Cell-Differentiation, IgD, IgM, Immunoglobulins-Surface, Lipopolysaccharides: ai, Lymphocyte-Transformation: de, Mercaptoethanol, Mice, Mice-Inbred-C3H: im, SUPPORT-U-S-GOVT-P-H-S

First Page

2401

Last Page

2405

JAX Source

Proc-Natl-Acad-Sci-USA. 1978 May; 75(5):2401-5.

Abstract

The effects of various anti-Ig antibodies on different B lymphocyte functions were investigated. With the proper accessory cofactor(s) derived from serum, anti-IgM antibodies induced a vigorous proliferative response in normal adult murine B cells, while polyspecific anti-Ig and anti-IgD had no effect. Without the required cofactor, all three anti-Ig antibodies were inhibitory for mitogenic responses. All three anti-Ig antibodies were also inhibitory for mitogen-induced antibody responses with or without the cofactor. Even with the required cofactor, neonatal B cells as well as adult C3H/HeJ B cells were not triggered into proliferation by anti-IgM. Finally, the cofactor required for anti-IgM-triggered mitogenesis was shown to be generated from serum by 2-mercaptoethanol and to be approximately 65,000 in molecular weight. These results indicate that, for at least some responses, B lymphocyte surface IgM molecules are involved in both triggering and suppression, depending both on the developmental state of the B cell and the presence or absence of accessory influences. In these experiments, IgD gave evidence only of being suppressive.

Please contact the Joan Staats Library for information regarding this document.

Share

COinS