Faculty Research 1970 - 1979
Endocrine pancreatic cells of postnatal "diabetes" (db) mice in cell culture.
Document Type
Article
Publication Date
1979
Keywords
Cell-Differentiation, Comparative-Study, Cytoplasmic-Granules: ul, Diabetes-Mellitus-Experimental: me, pa, Epinephrine, Glucagon: bi, Insulin: bi, Islands-of-Langerhans: me, ul, Mice, Mice-Inbred-Strains, Organoids: ul, SUPPORT-U-S-GOVT-P-H-S, Tissue-Culture, Tolbutamide
First Page
507
Last Page
521
JAX Source
In-Vitro. 1979 Jul; 15(7):507-21.
Abstract
Ultrastructural characteristics as well as secretory and biosynthetic behavior of monolayer pancreatic cell cultures established from 4-day-old C57BL/KsJ misty diabetic (m db/m db) mice have been studied in comparison to normal littermate controls. Hypersecretion of glucagon by alpha-cells from BL/Ks misty diabetic mice after 2 days in vitro was found to precede any hyperfunction of the insulin-secreting beta-cells. The increased level of glucagon-release in BL/Ks cell cultures from diabetic mice was accompanied by a greatly enhanced level of incorporation of [3H]tryptophan into glucagon-like molecules whose specific radioactivity was up to 15-fold higher than that observed in cultures from genetic controls. The finding of an alpha-cell dysfunction in cultures established from preweaning diabetic BL/Ks mice suggests that glucagon could play an early role in shaping the events that culminate in the expression of frank diabetes in this inbred strain.
Recommended Citation
Leiter EH,
Coleman DL,
Eppig JJ.
Endocrine pancreatic cells of postnatal "diabetes" (db) mice in cell culture. In-Vitro. 1979 Jul; 15(7):507-21.