Document Type

Article

Publication Date

1-1-2026

Keywords

JMG, Humans, Dental Pulp, Fibroblasts, Angiotensin II, Interleukin-1beta, Cells, Cultured, Porphyromonas gingivalis, Flow Cytometry, Lipopolysaccharides, Renin-Angiotensin System, Inflammation Mediators, Adult, Escherichia coli, Child, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Time Factors

JAX Source

J Appl Oral Sci. 2026;34:e20250822.

ISSN

1678-7765

PMID

41919869

DOI

https://doi.org/10.1590/1678-7765-2025-0822

Abstract

OBJECTIVE: This study aimed to evaluate the capacity of fibroblasts from permanent and deciduous dental pulp to express inflammatory mediators when exposed to bacterial and inflammatory antigens. It also sought to assess the presence of components of the renin-angiotensin system (RAS) and the potential modulatory role of ANG-II in this response.

METHODOLOGY: Fibroblasts were cultured from the pulp of permanent and deciduous teeth obtained from three adult and four pediatric donors with informed consent. Cells were stimulated with Porphyromonas gingivalis and Escherichia coli lipopolysaccharides (LPS) either alone or in combination with ANG-II and, in some cases, with IL-1β. Gene expression of RAS components and various inflammatory mediators was analyzed by qRT-PCR. ANG-II receptor expression was quantified by flow cytometry.

RESULTS: The results showed that both bacterial antigens and IL-1β significantly upregulated the expression of genes encoding key inflammatory mediators. However, ANG-II, either alone or in combination with other antigens, failed to alter the expression levels of these mediators.

CONCLUSION: These findings clearly show that ANG-II has no influence on the expression of inflammatory mediators by human dental pulp fibroblasts under the tested conditions.

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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