Faculty Research 1970 - 1979
Cytoplasmic and nuclear protein synthesis in preimplantation mouse embryos.
Blastocyst: me, Cell-Nucleus: me, Cytoplasm: me, Electrophoresis-Polyacrylamide-Gel, Mice, Proteins: bi, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S
J Embryol Exp Morphol 1979 Aug;52:209-25
Cytoplasmic and nuclear proteins synthesized by mouse embryos at different stages of preimplantation development were analyzed by two-dimensional polyacrylamide gel electrophoresis. Several nuclear-specific proteins (i.e. proteins more abundant in the nucleus than in the cytoplasm) and numerous cytoplasmic-specific proteins were observed. The trends of changes in the nuclear and cytoplasmic protein synthesis are similar. Moderate changes occur between the unfertilized egg and the zygote. Striking changes characterized by the disappearance of numerous major oocyte-specific proteins and the appearance of a large number of new, stage-specific proteins occur between the zygote and the 4- to 8-cell stages. In contrast, between the 4- and 8-cell and early blastocyst periods, only a few new proteins appear, and a small number of oocyte-specific or other stage-specific proteins disappear. Minor differences in protein synthesis were observed between the trophoblast and inner cell mass.
Howe, C C. and Solter, D, " Cytoplasmic and nuclear protein synthesis in preimplantation mouse embryos." (1979). Faculty Research 1970 - 1979. 1094.