Faculty Research 1970 - 1979

Non-specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas.

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Cytotoxicity-Tests-Immunologic, DNA: bi, Fibrosarcoma: ci, im, Immunity-Cellular, Lymphoma: im, me, Mice, Mice-Inbred-CBA, Mice-Inbred-C57BL, Mice-Inbred-DBA, Neoplasm-Transplantation, Neoplasms-Experimental: im, Sarcoma-Experimental: im, me, Spleen: im, Transplantation-Isogeneic

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Br-J-Cancer. 1977 Jul; 36(1):35-40.


Spleen cells collected from mice bearing transplanted chemically induced syngeneic fibrosarcomas non-specifically inhibited DNA synthesis of sarcoma and lymphoma target cells in vitro. Splenocytes from mice hyper-immunized against a syngeneic sarcoma specifically inhibited DNA synthesis of the tumour used for immunization. The impairment of tumour-cell DNA synthesis was associated in vitro with cytostasis, and lysis of the target cells was not seen. Since treatment with anti-theta serum and complement did not impair cytostatic action of the spleen cells, and since thymus-deprived animals showed similar activity to normal mice, T lymphocytes were not involved in non-specific cytostasis. Removal of phagocytic adherent cells by carbonyl iron markedly inhibited the cytostatic activity of the spleen cells, suggesting a role in this reaction for cells of the monocyte-macrophage series. The presence of an actively growing sarcoma was a prerequisite for the expression of non-specific cytostasis, since surgical excision resulted in complete disappearance of this activity of spleen cells.

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