MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
Catalysis, Chromatography-Affinity, Electrophoresis-Polyacrylamide-Gel, Female, Glucuronidase: bi, ge, In-Vitro, Mice, Mice-Inbred-A, Oocytes: en, Ovum: en, RNA-Messenger: ge, ph, SUPPORT-U-S-GOVT-NON-P-H-S, Translation-Genetic, Xenopus
Proc Natl Acad Sci U S A 1977 Oct; 74(10):4462-5.
Catalytically active mouse beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 22.214.171.124) is formed when Xenopus oocytes are injected with mouse RNA enriched for poly(A)-containing mRNA sequences. With the RNA from androgen-induced kidneys, the efficiency of translation is comparable to that of endogenous Xenopus messenger, and the fidelity of translation is high. Detection of glucuronidase messenger by formation of a catalytically active product is several orders of magnitude more sensitive than detection by incorporation of isotopically labeled amino acids. As well as providing a sensitive technique for examining the regulation of gene expression, the system makes available an opportunity to study the regulation of post-translational polypeptide processing of a lysosomal enzyme.
Labarca, C and Paigen, K, " MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes." (1977). Faculty Research 1970 - 1979. 807.