Faculty Research 1970 - 1979

Comparison of benzo[a]pyrene metabolism by human peripheral blood lymphocytes and monocytes.

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Chromatography-High-Pressure-Liquid, Comparative-Study, Epoxy-Compounds, Flavones, Human, Hydrocarbons-Chlorinated, In-Vitro, Lymphocytes: de, me, Monocytes: de, me, Naphthalenes, SUPPORT-U-S-GOVT-P-H-S

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Cancer Lett 1978 Nov; 5(5):261-8.


Here we report a more sensitive high-pressure liquid chromatographic (HPLC) assay of benzo[a]pyrene (BP) metabolites elaborated by human monocytes and lymphocytes using only 10 X 10(6) cells for HPLC analysis. The major metabolites formed by both lymphocytes and monocytes were 3-hydroxy-BP, 9-hydroxy-BP and quinones. BP-dihydrodiols were also found by HPLC analysis, the major one being BP-7,8-dihydrodiol for both cell types. In addition, a peak slightly more polar than 9,10-dihydrodiol was formed by lymphocytes. The 7,8-diol peak was eliminated by the addition of 1,1,1-trichloropropene oxide to the incubation mixture. The presence of alpha-napthoflavone resulted in an overall decrease in metabolite production.

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