Faculty Research 1970 - 1979

Endocrine pancreatic cells of postnatal "diabetes" (db) mice in cell culture.

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Cell-Differentiation, Comparative-Study, Cytoplasmic-Granules: ul, Diabetes-Mellitus-Experimental: me, pa, Epinephrine, Glucagon: bi, Insulin: bi, Islands-of-Langerhans: me, ul, Mice, Mice-Inbred-Strains, Organoids: ul, SUPPORT-U-S-GOVT-P-H-S, Tissue-Culture, Tolbutamide

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In-Vitro. 1979 Jul; 15(7):507-21.


Ultrastructural characteristics as well as secretory and biosynthetic behavior of monolayer pancreatic cell cultures established from 4-day-old C57BL/KsJ misty diabetic (m db/m db) mice have been studied in comparison to normal littermate controls. Hypersecretion of glucagon by alpha-cells from BL/Ks misty diabetic mice after 2 days in vitro was found to precede any hyperfunction of the insulin-secreting beta-cells. The increased level of glucagon-release in BL/Ks cell cultures from diabetic mice was accompanied by a greatly enhanced level of incorporation of [3H]tryptophan into glucagon-like molecules whose specific radioactivity was up to 15-fold higher than that observed in cultures from genetic controls. The finding of an alpha-cell dysfunction in cultures established from preweaning diabetic BL/Ks mice suggests that glucagon could play an early role in shaping the events that culminate in the expression of frank diabetes in this inbred strain.

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