Faculty Research 1980 - 1989
Genome organization and polymorphism of the murine beta-glucuronidase region.
Document Type
Article
Publication Date
1988
Keywords
Cloning-Molecular, Comparative-Study, DNA-Restriction-Enzymes, Genes-Structural, Glucuronidase: ge, Haplotypes, Mice, Mice-Inbred-C57BL, Polymorphism-(Genetics), SUPPORT-U-S-GOVT-P-H-S
First Page
25
Last Page
31
JAX Source
Genomics 1988 Jan; 2(1):25-31.
Grant
GM31656
Abstract
Thirty-eight kilobases of mouse genomic DNA which surround and include the coding sequences for beta-glucuronidase has been mapped. Intron-exon arrangements were determined by hybridization of genomic sequences with cDNA clones, and minimum estimates of gene length (11-17 kb) and intron number were obtained. Only a single gene was observed when genomic DNA was probed with subclones containing beta-glucuronidase coding sequence; there was no evidence of duplicated or pseudogenes. However, sequences distal to the 3' end of the gene are present elsewhere in the genome in a limited number of copies. Eight haplotypes of the beta-glucuronidase region with differing regulatory genotypes were compared for restriction fragment polymorphisms. Surprisingly little was found, considering the diverse origin of the haplotypes. Two of the polymorphisms that were found may be correlated with regulatory phenotypes. A BamHI site is missing from the CS and CL haplotypes that share regulatory properties, and a 0.2-kb insertion is consistently present in haplotypes showing increased response to induction by androgens in kidney.
Recommended Citation
Moore KJ,
Paigen K.
Genome organization and polymorphism of the murine beta-glucuronidase region. Genomics 1988 Jan; 2(1):25-31.