Ultraviolet shadowing nucleic acids on nylon membranes.

Authors

S J. Thurston
J D. Saffer

Document Type

Article

Publication Date

1989

Keywords

Nucleic-Acids: an, Nylons: re, SUPPORT-NON-U-S-GOVT, Ultraviolet-Rays

First Page

41

Last Page

42

JAX Location

1756

Abstract

We describe a method for the direct visualization of nucleic acids on nylon membranes. Nylon is weakly fluorescent under short wave ultraviolet light allowing membrane-bound nucleic acids to be detected with a sensitivity of 10 ng. This procedure involves no staining or destaining of the gels prior to transfer, does not require duplicate sample lanes or blots, and does not interfere with transfer of the nucleic acid to the membrane or subsequent hybridization.

Recommended Citation

Thurston SJ, Saffer JD. Ultraviolet shadowing nucleic acids on nylon membranes. . 1989; ():41