Faculty Research 1980 - 1989

Genetic and tissue-specific variation in the expression of a closely linked murine multigene family on chromosome 15 that encodes salivary and lacrimal proteins.

Document Type

Article

Publication Date

1989

Keywords

Chromosome-Mapping, Cloning-Molecular, Comparative-Study, DNA: ge, Gene-Expression, Genes-Reiterated, Genes-Structural, Hamsters, Hybrid-Cells: me, Immunoblotting, Linkage-(Genetics), Mice, Mice-Inbred-Strains: ge, Organ-Specificity, Phenotype, Proteins: ge, RNA: ge, Salivary-Proteins: ge, Species-Specificity, Submandibular-Gland: me, SUPPORT-U-S-GOVT-P-H-S, Tears: an, Translation-Genetic, Variation-(Genetics)

First Page

613

Last Page

637

JAX Location

1924

JAX Source

Biochem Genet 1989 Oct; 27(9-10):613-37.

Grant

GM18684, CA33093, GM30248

Abstract

The murine submandibular gland (SMG) produces a novel class of highly acidic salivary proteins encoded by one or more highly abundant mRNA transcripts. In inbred mice, these transcripts are encoded by members of a multigene family comprising approximately 8-12 homologues. Most, and probably all, of these homologues are clustered at a new locus near belted (bt) on chromosome 15, which we designate Spt (salivary protein). Although physically closely linked, Spt genes differ in their patterns of expression both in strains of mice and in their tissues. One gene, Spt-1, is expressed at high levels in the SMG of all inbred strains examined. This gene is also expressed at significant levels in the lacrimal gland. A second gene, Spt-2, appears to be present as a single copy in some strains and as two copies in others. This gene is expressed at high levels only in the SMG of those strains carrying two copies, and Spt-2 mRNA is not detectable in the SMG of strains carrying only one copy. In contrast to Spt-1, the Spt-2 gene is not expressed at detectable levels in the lacrimal gland.

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