Faculty Research 1980 - 1989
Mouse Mos protooncogene product is present and functions during oogenesis.
Document Type
Article
Publication Date
1989
Keywords
Base-Sequence, Cells-Cultured, Female, Meiosis, Mice, Molecular-Sequence-Data, Oligonucleotide-Probes, Oocytes: cy, ph, Oogenesis, Proto-Oncogene-Proteins: ge, ph, Proto-Oncogenes, Support-U, S, -Gov't-P, H, S, Xenopus
First Page
5395
Last Page
5399
JAX Source
Proc Natl Acad Sci USA 1989 Jul; 86(14):5395-9.
Grant
NO1-CO-74101, HD20575
Abstract
We have identified the mouse Mos-encoded protein product, p39mos, in maturing mouse oocytes and have shown that it is indistinguishable from the product expressed in Mos-transformed NIH 3T3 cells. p39mos is detected in oocytes arrested in the first meiotic prophase, during germinal-vesicle breakdown, metaphase I, anaphase I, and in ovulated eggs. We show that microinjection of three different Mos antisense (but not sense) oligodeoxyribonucleotides into germinal vesicle-stage oocytes prevents first polar-body emission and therefore interrupted the normal progression of meiosis. These results show that in mouse oocytes, as in the amphibian Xenopus [Sagata, N., Oskarsson, M., Copeland, T., Brumbaugh, J. & Vande Woude, G.F. (1988) Nature (London) 335, 519-525], the product of Mos is necessary for normal meiotic maturation.
Recommended Citation
Paules RS,
Buccione R,
Moschel RC,
Vande W,
Eppig JJ.
Mouse Mos protooncogene product is present and functions during oogenesis. Proc Natl Acad Sci USA 1989 Jul; 86(14):5395-9.