Faculty Research 1980 - 1989
Localization of nucleic acid sequences by EM in situ hybridization using colloidal gold labels.
Document Type
Article
Publication Date
1989
Keywords
Base-Sequence, Chromosome-Mapping: mt, DNA, Gold: du, Mice: ge, Microscopy-Electron, Nucleic-Acid-Hybridization, RNA, SUPPORT-U-S-GOVT-P-H-S, Xenopus-laevis: ge
First Page
197
Last Page
204
JAX Source
Am J Anat 1989 Jun-Jul;185(2-3):197-204
Grant
GM23241, GM07311
Abstract
It is possible to combine hybridization to specimens on electron-microscope grids of nucleic-acid probes labelled nonisotopically with immunogold detection of hybrid sites to map the position of target sequences rapidly and precisely. The basic technique is described, and examples are provided to illustrate the types of questions which can be approached in the general area of higher-order chromosome organization and function. A combination of two differentially labelled probes and two different-sized gold particles permits the simultaneous detection of closely linked or interspersed sequences.
Recommended Citation
Hamkalo BA,
Narayanswami S,
Lundgren K.
Localization of nucleic acid sequences by EM in situ hybridization using colloidal gold labels. Am J Anat 1989 Jun-Jul;185(2-3):197-204