Faculty Research 1980 - 1989

Deletions in a recombinant retrovirus genome associated with its expression in embryonal carcinoma cells.

Document Type

Article

Publication Date

1989

Keywords

DNA-Viral: an, Genes-Viral, Moloney-Leukemia-Virus: ge, Proviruses: ge, Recombination-Genetic, Repetitive-Sequences-Nucleic-Acid, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Tumor-Cells-Cultured, Tumor-Stem-Cells: mi

First Page

4431

Last Page

4433

JAX Source

J Virol 1989 Oct;63(10):4431-3

Grant

CA39652

Abstract

We analyzed embryonal carcinoma cell lines infected with a recombinant Moloney murine leukemia virus. Lines that carried but did not express the neo gene retained a provirus of LTR-gag-pol-neo-LTR, where LTR is a long terminal repeat, whereas all G418-resistant lines deleted regions that included the primer binding site and the splicing donor site. This suggested the presence of multiple inhibitory elements.

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