Faculty Research 1980 - 1989

A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on chromosome 12.

Document Type

Article

Publication Date

1981

Keywords

Antibodies-Monoclonal: im, Antigens-Surface: ge, im, Cell-Line, Chromosomes-Human-6-12: ul, Fibrosarcoma: im, Human, Immunodiffusion, Immunosorbent-Techniques, Linkage-(Genetics), Mice, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Teratoma: im

First Page

435

Last Page

443

JAX Source

Somatic Cell Genet 1981 Jul;7(4):435-43

Grant

CA18470, GM07511, CA10815

Abstract

A murine monoclonal antibody 602-29, subclass IgG1, that recognizes an antigenic determinant expressed by most human cells is described. Immunoprecipitation and sodium dodecyl sulfate-gel electrophoresis analysis indicate that the antigenic determinant is carried by a protein with an apparent molecular weight of 21,000. The antigen is expressed by human-mouse somatic cell hybrids, and analysis of segregants that have lost human chromosomes indicates that the gene controlling expression of the 602-29 antigen is on chromosome 12.

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