Faculty Research 1980 - 1989

Hematopoietic stem cell function in motheaten mice.

Document Type

Article

Publication Date

1983

Keywords

Autoimmune-Diseases: bl, mo, Bone-Marrow: tr, Cell-Differentiation, Colony-Forming-Units-Assay, Female, Hematopoiesis: re, Hematopoietic-Stem-Cells: cy, Lung: pa, Male, Mice, Mice-Mutant-Strains, Radiation-Chimera, Spleen: tr, SUPPORT-U-S-GOVT-P-H-S

First Page

667

Last Page

680

JAX Source

Exp-Hematol. 1983 Aug; 11(7):667-80.

Grant

CA20408

Abstract

Mice homozygous for the autosomal recessive mutation "motheaten: have normal numbers of multipotential hematopoietic stem cells in the bone marrow and spleen as determined by spleen colony assay. Histologic examination shows no qualitative abnormality in morphology of stem cell colonies in recipients of bone marrow or spleen cells from motheaten mice. Despite the apparently normal ontogeny, distribution, and differentiative capacity of CFU stem cells, bone marrow and spleen cells from motheaten mice fail to save congenic +/+ lethally gamma-irradiated hosts. This impaired lifesparing capacity is not due to defective self-renewal but appears to be due in part to pulmonary hemorrhage from alveolar capillaries in the gamma-irradiated hosts. Treatment of motheaten mice with 500 R gamma-irradiation followed by reconstitution with normal bone marrow cells increases the lifespan of this mutant to 10 months of age. The early onset of pneumonitis and subsequent short lifespan of motheaten mice is determined at the level of progenitor cells in the bone marrow.

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