A recombinant DNA plasmid which inhibits bacteriophage T7 reproduction in escherichia coli.

Authors

J C. Stone
R D. Smith
R C. Miller

Document Type

Article

Publication Date

1983

Keywords

DNA-Bacterial: ge, DNA-Recombinant: me, DNA-Viral: ge, Escherichia-Coli: ge, Kinetics, Plasmids, SUPPORT-NON-U-S-GOVT, T-Phages: ge, Transcription-Genetic, Translation-Genetic, Virus-Replication

First Page

1615

Last Page

1622

JAX Source

J-Gen-Virol. 1983 Jul; 6(P):1615-22.

Abstract

Escherichia coli plasmids carrying a DNA restriction fragment corresponding to the extreme right end of the T7 bacteriophage genome render cells incompetent to support reproduction of wild-type T7. Processing of intracellular concatemeric phage DNA and cell lysis are perturbed by one such plasmid, pRS148. The results are discussed with reference to the possibility that the right end of the T7 genome encodes a lysis-related function.

Recommended Citation

Stone JC, Smith RD, Miller RC. A recombinant DNA plasmid which inhibits bacteriophage T7 reproduction in escherichia coli. J-Gen-Virol. 1983 Jul; 6(P):1615-22.