Faculty Research 1980 - 1989

Different tissue concentrations and rates of synthesis of dolichol, dolichyl acyl esters, and dolichyl phosphate in mouse testes and preputial glands.

Document Type

Article

Publication Date

1983

Keywords

Comparative-Study, Diterpenes: me, Dolichol: aa, bi, me, Dolichol-Phosphates: bi, me, Exocrine-Glands: me, Male, Mice, Mice-Inbred-C57BL, Polyisoprenyl-Phosphates: me, SUPPORT-U-S-GOVT-P-H-S, Testis: me

First Page

512

Last Page

518

JAX Source

Biochem-Biophys-Res-Commun. 1983 Jan 27; 110(2):512-8.

Grant

GM29241

Abstract

The rates of synthesis and tissue concentrations of dolichol derivatives differ markedly in testes and preputial glands from adult C57BL/6J mice. In testes, the rates of free dolichol, dolichyl acyl esters, and dolichyl phosphate synthesis were approximately 5,000, 4,000 and 2,000 dpm/3h/g, respectively. Comparable rates for preputial glands were 12,000, 60,000 and 0 dpm/3h/g. Thus in testes, dolichyl phosphate represented 15-20% of total dolichol synthesis, whereas no de novo dolichyl phosphate synthesis was detected in preputial glands. In testes, free dolichol was the predominant derivative synthesized; in preputial glands, 80% of dolichol synthesized was esterified to fatty acids. The concentration of total dolichol (free alcohol plus acyl esters) was 160 micrograms/g tissue in testes and 1270 micrograms/g tissue in preputial glands. In both tissues, 85-90% of dolichol was esterified to fatty acids, and no dolichyl phosphate was detected.

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