Faculty Research 1980 - 1989

Identification and purification of a cell surface glycoprotein mediating intercellular adhesion in embryonic and adult tissue.

Document Type

Article

Publication Date

1983

Keywords

Cell-Adhesion, Cell-Communication: de, Cell-Line, Embryo: cy, Female, Fluorescent-Antibody-Technique, Glycoproteins, Human, Immune-Sera, Mammary-Neoplasms-Experimental: an, Mice, Molecular-Weight, Pregnancy, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Surface-Properties

First Page

455

Last Page

466

JAX Source

Cell 1983 Sep;34(2):455-66

Grant

CA27909, CA32311, HD15663, +

Abstract

An antiserum against material shed into serum-free medium by MCF-7 human mammary carcinoma cells (anti-SFM II) disrupts cell-cell interactions in murine mammary tumor epithelial cells (MMTE). We now report purification of an 80 kd glycoprotein (GP80) from SFM of MCF-7 mammary carcinoma cells that blocks the activity of anti-SFM II. Anti-SFM II also inhibits compaction of eight-cell mouse embryos, and purified GP80 blocks this reaction. An antiserum against purified GP80 (anti-GP80) has all adhesion-disrupting activities displayed by anti-SFM II. It recognizes one band at 80 kd in SFM and a 120 kd band in detergent extracts of epithelial but not fibroblastic cells. In immunofluorescence studies it is restricted to sites of cell-cell interaction in cultured epithelial cells. Thus a cell surface glycoprotein of 120 kd, the medium form of which is approximately 80 kd, which is neither species nor tissue specific, is expressed at early stages of mammalian development and is found on epithelia.

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