Faculty Research 1980 - 1989
Spontaneous and induced leukemias of myeloid origin in recombinant inbred BXH mice.
Document Type
Article
Publication Date
1984
Keywords
Antigens-Surface: an, Cell-Line, DNA-Neoplasm: an, DNA-Viral: an, Leukemia-Experimental: fg, im, Mice, Mice-Inbred-Strains, Mouse-Leukemia-Viruses: ip, Phenotype, RNA-Neoplasm: an, RNA-Viral: an, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, T-Lymphocytes: im
First Page
586
Last Page
594
JAX Location
3
JAX Source
J-Virol. 1984 Sep; 51(3):586-94.
Grant
CA31102, CA37283, CA32630
Abstract
BXH-2 recombinant inbred (RI) mice produce high titers of B-ecotropic murine leukemia virus beginning early in life and have a high incidence of non-T-cell leukemias that occur before 1 year of age. The leukemias that develop are in some cases associated with hind limb paralysis. In addition, a dualtropic mink cell focus-forming virus has been isolated from leukemic cells of BXH-2 mice. Immunological and cytochemical characterization of the BXH-2 leukemias showed that they are of the myeloid lineage. To assess the oncogenicity of the BXH-2 viruses, newborn mice of several BXH RI strains were inoculated at birth with biologically cloned B-ecotropic or mink cell focus-forming murine leukemia virus. These studies demonstrated that the B-ecotropic virus can induce myeloid leukemias in other BXH RI strains, whereas the dualtropic mink cell focus-forming isolates were nononcogenic in the strains tested. DNA-DNA reassociation analysis indicated that the organotropism of the B-ecotropic murine leukemia virus is confined to lymphoid tissues. Southern analysis of tumor DNAs showed that there was amplification of ecotropic virus-specific sequences in BXH-2 myeloid tumors and in all leukemias induced in other BXH RI strains by inoculation of the BXH-2 B-ecotropic virus. Although B-ecotropic virus is expressed in central nervous tissues of paralyzed BXH-2 mice, we were unable to induce the disorder in several BXH RI strains inoculated intracranially at birth with either the B-ecotropic or dualtropic virus. These results suggest that the paralysis that occurs in BXH-2 mice is due to the infiltration of leukemic cells into the central nervous system.
Recommended Citation
Bedigian HG,
Johnson DA,
Jenkins NA,
Copeland NG,
Evans R.
Spontaneous and induced leukemias of myeloid origin in recombinant inbred BXH mice. J-Virol. 1984 Sep; 51(3):586-94.