Spontaneous temperature-sensitive mutations in bacteriophage T7.

Authors

J C. Stone
R C. Miller

Document Type

Article

Publication Date

1985

Keywords

Genes-Viral, Genetic-Complementation-Test, Mutation, SUPPORT-NON-U-S-GOVT, T-Phages: ge, Temperature

First Page

886

Last Page

888

JAX Source

J-Virol. 1985 Jun; 54(3):886-8.

Abstract

Attempts to recover temperature-sensitive mutations affecting genes 13 and 14 (virion proteins) in bacteriophage T7 by analysis of amber revertants were confounded by the frequent occurrence of spontaneous temperature-sensitive mutations in other genes. These incidental temperature-sensitive mutations are physically distinct from but may be functionally related to genes 13 and 14, as shown by complementation and recombination studies. The possibility that these incidental temperature-sensitive mutations represent secondary-site suppressors of the pseudonormal suppressed amber products is discussed.

Recommended Citation

Stone JC, Miller RC. Spontaneous temperature-sensitive mutations in bacteriophage T7. J-Virol. 1985 Jun; 54(3):886-8.