Faculty Research 1980 - 1989
Linkage of a 7S RNA sequence and kappa light chain genes in the mouse.
Document Type
Article
Publication Date
1985
Keywords
Base-Sequence, Cell-Line, DNA-Restriction-Enzymes: me, Genes-Structural, Immunoglobulins-Kappa-Chain: ge, Immunoglobulins-Light-Chain: ge, Linkage-(Genetics), Mice, Mice-Inbred-A, Mice-Inbred-AKR, Mice-Inbred-BALB-C, Mice-Inbred-CBA, Mice-Inbred-C3H, Mice-Inbred-C57BL, Mice-Inbred-DBA, Mice-Inbred-NZB, Plasmacytoma: fg, im, Recombination-Genetic, RNA-Ribosomal: me, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
471
Last Page
481
JAX Source
Immunogenetics. 1985; 22(5):471-81.
Grant
GM18684, CA33093, AI13797, +
Abstract
A mouse 7S RNA cDNA plasmid clone was employed to identify and map DNA restriction fragment variants using recombinant inbred (RI) and congenic mouse strains. More than a dozen such restriction variants were identified and mapped to different regions of the mouse genome. One such variant, designated Rn7s-6, showed close linkage to the Ly-2,3-Igk-V (T lymphocyte antigens 2 and 3, kappa immunoglobulin variable region) cluster of markers on chromosome 6. No recombinants were detected among three of these markers in 59 RI strains. On the basis of these data, the Rn7s-6 sequence may be placed within 1.3 centimorgans of Ly-3 and one of the Igk-V-region markers, Igk-Ef1. Two mouse stocks with previously identified crossovers within the Ly-2,3-Igk-V region were used to sublocalize Rn7s-6. The results are consistent with the gene order (Ly-2, Ly-3)-(Rn7s-6, Igk-Ef1)-Igk-Ef2. Several mouse plasmacytomas, known to have various parts of the kappa chain complex deleted, retain the Rn7s-6 sequence. The Rn7s-6 variant is a plus/minus variant; no sequence allelic to Rn7s-6 is found in inbred strains that share the Ly-3a-Igk-Ef1a haplotype.
Recommended Citation
Taylor BA,
Rowe L,
Gibson DM,
Riblet R,
Yetter R,
Gottlieb PD.
Linkage of a 7S RNA sequence and kappa light chain genes in the mouse. Immunogenetics. 1985; 22(5):471-81.