Faculty Research 1980 - 1989
Tumor location using F(ab')2 mu from a monoclonal IgM antibody: pharmacokinetics.
Document Type
Article
Publication Date
1985
Keywords
Antibodies-Monoclonal: du, Antigens-Neoplasm: im, Glycolipids: im, IgM, Immunoglobulins-Fab, Iodine-Radioisotopes: du, Kinetics, Mice, Mice-Inbred-BALB-C, Neoplasms-Experimental: ri, Plasmacytoma: ri, SUPPORT-U-S-GOVT-NON-P-H-S, SUPPORT-U-S-GOVT-P-H-S, Teratoma: ri, Time-Factors
First Page
283
Last Page
292
JAX Source
J Nucl Med 1985 Mar;26(3):283-92
Grant
HD12487, CA31784
Abstract
A monoclonal IgM antibody (anti-SSEA-1) and its divalent antigen-binding peptic fragment [F(ab')2 mu] were compared as in vivo tumor localization reagents in mouse teratocarcinomas. F(ab')2 mu is cleared more rapidly than whole antibody from the whole body, blood, and all tested organs (t1/2 for whole antibody approximately 18 hr; t1/2 for F(ab')2 mu, 12 hr). A corresponding average improvement in tumor-to-tissue ratio is observed 48 hr after injection and earlier. However, the affinity of the F(ab')2 mu for antigen is much lower, and a smaller fraction of the antibody fragment is retained in the tumor than with whole antibody. The fragment was not retained by animals bearing nonantigenic tumors.
Recommended Citation
Ballou B,
Reiland J,
Levine G,
Knowles B,
Hakala TR.
Tumor location using F(ab')2 mu from a monoclonal IgM antibody: pharmacokinetics. J Nucl Med 1985 Mar;26(3):283-92