Faculty Research 1980 - 1989
A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on human chromosome 1.
Document Type
Article
Publication Date
1985
Keywords
Antibodies-Monoclonal: du, Antigenic-Determinants: ge, Antigens-Surface: ge, Cell-Line, Cell-Separation, Chromosome-Mapping, Chromosomes-Human-1-3, Flow-Cytometry, Human, Hybridomas, Mice, Precipitin-Tests, Radioimmunoassay, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S
First Page
31
Last Page
39
JAX Source
Ann Hum Genet 1985 Jan;49 ( Pt 1):31-9
Grant
CA29894, CA18470
Abstract
An antigen expressed by most human cells, but not erythrocytes, has been defined by a murine monoclonal antibody, TRA-2-10. This antigen is expressed on the surface of human-mouse somatic cell hybrids, and segregation analysis indicates that it is controlled by a gene located on human chromosome 1. From lysates of most human cells, surface-labelled with 125I, TRA-2-10 immunoprecipitates two polypeptides with molecular weights in the range of about 55 000 to 73 000 depending upon the cell line. Since the TRA-2-10 polypeptides from a fibroblast cell strain and a hepatoma cell line from one individual differ, we conclude that the observed difference in molecular weight has an epigenetic origin.
Recommended Citation
Andrews PW,
Knowles BB,
Parkar M,
Pym B,
Stanley K,
Goodfellow PN.
A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on human chromosome 1. Ann Hum Genet 1985 Jan;49 ( Pt 1):31-9