Faculty Research 1980 - 1989

Title

A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on human chromosome 1.

Document Type

Article

Publication Date

1985

Keywords

Antibodies-Monoclonal: du, Antigenic-Determinants: ge, Antigens-Surface: ge, Cell-Line, Cell-Separation, Chromosome-Mapping, Chromosomes-Human-1-3, Flow-Cytometry, Human, Hybridomas, Mice, Precipitin-Tests, Radioimmunoassay, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S

JAX Source

Ann Hum Genet 1985 Jan;49 ( Pt 1):31-9

Grant

CA29894, CA18470

Abstract

An antigen expressed by most human cells, but not erythrocytes, has been defined by a murine monoclonal antibody, TRA-2-10. This antigen is expressed on the surface of human-mouse somatic cell hybrids, and segregation analysis indicates that it is controlled by a gene located on human chromosome 1. From lysates of most human cells, surface-labelled with 125I, TRA-2-10 immunoprecipitates two polypeptides with molecular weights in the range of about 55 000 to 73 000 depending upon the cell line. Since the TRA-2-10 polypeptides from a fibroblast cell strain and a hepatoma cell line from one individual differ, we conclude that the observed difference in molecular weight has an epigenetic origin.

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