Faculty Research 1980 - 1989
Linkage of prion protein and scrapie incubation time genes.
Document Type
Article
Publication Date
1986
Keywords
Chromosome-Mapping, DNA-Restriction-Enzymes: du, Linkage-(Genetics), Mice, Mice-Inbred-Strains: ge, Polymorphism-(Genetics), Prions: ge, Scrapie: fg, pa, SUPPORT-NON-U-S-GOVT, SUPPORT-U-S-GOVT-P-H-S, Time-Factors
First Page
503
Last Page
511
JAX Location
1308
JAX Source
Cell. 1986 Aug 15; 46(4):503-11.
Grant
CA28231, NS22786, AG02132
Abstract
A single gene (Prn-i) that affects scrapie incubation period in mice has been identified. I/LnJ mice have a very long incubation period after inoculation of scrapie prions (200-385 days) and NZW/LacJ mice have a short one (113 +/- 2.8 days). (NZW X I/Ln)F1 hybrid mice had incubation times of 223 +/- 2.8 days indicating longer incubation times were dominant. Incubation periods in the backcross progeny of (NZW/LacJ X I/LnJ)F1 X NZW/LacJ segregated into two groups (64 mice, 130 +/- 1.1 d; 66 mice, 195 +/- 1.9 d) indicating single gene control. NZW/LacJ and 20 other inbred strains have the Prn-pa allele which is identified as a 3.8 kb Xbal fragment using a hamster PrP (prion protein) cDNA probe. I/LnJ and three other Prn-pb mouse strains have a 5.5 kb Xbal restriction fragment. Analysis of DNA from 66 backcross mice indicated Prn-i is tightly linked to Prn-p, the structural gene for PrP.
Recommended Citation
Carlson GA,
Kingsbury DT,
Goodman PA,
Coleman S,
Marshall ST,
DeArmond S,
Westaway D,
Prusiner SB.
Linkage of prion protein and scrapie incubation time genes. Cell. 1986 Aug 15; 46(4):503-11.